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Mutations of KXD/E motif further proved the essential function of the KXD/E motif in mediating Golgi localization of EMPs. Correct Golgi targeting should be essential for the normal functions of EMPs. A direct approach to prove this hypothesis is to perform mutant complementation assays by expression of either GFPEMPs or EMPs-GFP in mutants harboring mutations in the corresponding EMP allele to se

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