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E as a subunit vaccine against HSV-1 infection. DISCUSSION Structural, immunological, and functional studies of the HSV gH-gL complex have been hampered due to the lack of a suitable source of purified protein. Milligram amounts of a truncated mammalian form of HSV-1 gH-gL have now been obtained using a stably transfected L-cell line, HL-7, which secretes gHt and gL as a complex. In agreement with

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