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Nditioned medium, the nonadherent cells were washed off again, resulting in a population of cells with predominantly macrophage (>99 ) morphology as determined with the modified Wright's stain. Adherent AM monolayers were infected with J2 virus supernatants mixed with DMEM conditioned media and polybrene. After 24 h, excess virus was removed and cells were exposed to a second round of J2 infection

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