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5 and 0.1% Triton X-100) #links# and then to lysis buffer. Following overnight incubation at 4��C with slight agitation, the beads were collected by centrifugation and boiled 5?min in tricine sample buffer (Bio-Rad). Twenty-five microlitres of apical membrane proteins was separated on 4�C20% polyacrylamide gels (Bio-Rad), transferred to PVDF membranes, and immunoblotted for MRP2 (Santa Cruz) and t

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