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Ermined by trypan blue dye exclusion. Doubling time (mean generation time = mgt) was calculated according the formula: N = N02T/mgt. N is the number of cells at any time T; N0, is the number of cells at an initial point. Detection of macrophage-associated cell-surface antigens by flow cytometry assay Expression of CD11b (Mac-1) and F4/80 antigens was demonstrated by immunofluorescence staining of

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