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Ith cold PBS. Cell counts and viability were determined by using a hemocytometer and trypan blue dye exclusion. Cytocentrifuge preparations were stained with Diff-Quik, a modified Wright-Giemsa stain to allow differential analysis. All BAL samples contained greater than 95 AMs. AMs were adjusted to 1 ?106 cells/ml in DMEM conditioned medium (CM), which contained DMEM medium supplemented with 15

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